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casein. 2 % tyrosine. For biuret trial ; 1 milliliter of each solution in 1,2,3 and 5 was placed except casein and casein was put and 1 milliliter H2O was added in trial tubing 4. 7-10 beads of 2.5 M NaOH was added and 2 beads of 5 % Cu sulphate solution was added on each of the samples. Observations were recorded. Take 0.5gm powdered sodium bi-sulphate (NaHSO 4) or potassium bi-sulphate (KHSO 4) in a clean dry test tube, add 3 to 4 drops of sample. Mix thoroughly and heat.
Casein is a calcium rich, slow digesting protein source that is anti-catabolic in nature. It remains a staple supplement for top bodybuilders, athletes and fitness enthusiasts.
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A formula for biuret reagent is (per liter final volume) 9 gm Sodium potassium tartrate (f.w. 282.22), 3 gm Copper sulfate x 5 H2O (f.w. 249.68), 5 gm Potassium iodide (166.0), all dissolved in order in 400 ml 0.2 M NaOH (f.w. 40.0) before bringing to final volume. The volume can be scaled up or scaled down of course. Bmw e90 320i intake manifold removal.
The Biuret method. is based on the complexation of Cu2+ to functional groups. in the protein's peptide bonds as shown in Figure 1. The standards and sample were analyzed using the Biuret method included in Thermo Scientific VISIONcollect software with biological tests.Biuret Test for Proteins: Biuret reagent is a light blue solution which turns purple when mixed with a solution containing protein. The purple color is formed when copper ions in the biuret reagent react with the peptide bonds of the polypeptide chains to form a complex. Label a set of tubes with a wax pencil.